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Metabolic Engineering

Biofuel Production in Ralstonia eutropha H16

My post-doctoral work at LBL/JBEI with Harry Beller, Steven Singer, and Nathan Hillson focuses on metabolically engineering the -proteobacterium Ralstonia eutropha H16 to produce hydrocarbons and methyl ketones from H2 and CO2 using advanced synthetic biology tools. Strain performance is evaluated with state of the art analytics. This project is funded by the ARPA-E electrofuels program.

For more information, please see the official project website.

Bacterial Physiology

Physiology of Shewanella oneidensis MR-1 and Vibrio cholerae Biofilms

Image of Shewanella oneidensis biofilm
Shewanella oneidensis biofilm

My graduate work in the Spormann Lab at Stanford University focused on elucidating factors involved in formation and detachment of Shewanella oneidensis MR-1 and Vibrio cholerae biofilms. In particular I was interested in the regulation of the mxd operon in S. oneidensis MR-1. The mxd genes were previously identified by Tn5 mutagenesis and found to play a role in biofilm formation of this organism. An understanding of factors controlling stability and persistence of lab-grown biofilms is important, so that this knowledge can be applied to environmentally and medically relevant biofilms.